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KMID : 1007520230320010083
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Food Science and Biotechnology
2023 Volume.32 No. 1 p.83 ~ p.90
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Anti-biofilm effects of sinomenine against Staphylococcus aureus
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Yum Su-Jin
Jeong Hee-Gon
Kim Seung-Min
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Abstract
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Staphylococcus aureus is a gram-positive foodborne pathogen capable of forming strong biofilms. This study identified that anti-biofilm natural compound against S. aureus. Sinomenine, a natural compound, showed significantly reduced biofilm formation (31.97?39.86%), but no effect on bacterial growth was observed. The dispersion of preformed biofilms was observed by confocal laser scanning microscopy (CLSM). qRT-PCR revealed that sinomenine treatment significantly up-regulated agrA by 3.8-fold and down-regulated icaA gene by 3.1-fold. These indicate that sinomenine treatment induces biofilm dispersal due to cell-cell adhesion, polysaccharide intercellular adhesin (PIA), and phenol-soluble modulin (PSM) peptides production. Our results suggest that sinomenine can be used as a promising agent for effectively controlling biofilm formation and dispersion, thereby making S. aureus more susceptible to the action of antimicrobial agents.
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KEYWORD
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Staphylococcus aureus, Biofilm, Sinomenine, Natural compound
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